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Magnesium chloride solution, 25 mM, sterile, for molecular biology, 1 mlMagnesium chloride solution, 25 mM, sterile, for molecular biology, Molar mass (M) 95,22 g mol, Density (D) 1,003 g cm, Boiling point (bp) ~100 C, Storage temp. +4 C, Empirical formula MgCl2 Sterile In a PCR reaction, cofactors such as divalent cations (MgCl2) are needed for an optimal process. Magnesium interacts with the DNA template, dNTPs and the polymerase in a PCR reaction. The MgCl2 concentration influences the productivity and accuracy of the
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Magnesium chloride solution, 25 mM, sterile, for molecular biology, Molar mass (M) 95,22 g/mol, Density (D) 1,003 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C, Empirical formula MgCl2

Sterile

In a PCR reaction, cofactors such as divalent cations (MgCl2) are needed for an optimal process. Magnesium interacts with the DNA template, dNTPs and the polymerase in a PCR reaction. The MgCl2 concentration influences the productivity and accuracy of the polymerase. It also influences the binding of the primers to the template.

Application:
  • Too high magnesium concentration can lead to reduced specificity and undesired PCR products
  • Too low a magnesium concentration can lead to no PCR product at all
  • Magnesium influences the melting point of double-stranded DNA

Magnesium chloride solution, 25 mM, sterile, for molecular biology, 1 ml

Item no : 65536401920
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