RPL10 (F34) Polyclonal Antibody

Catalogue Numbers: BS2549-50, BS2549-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P27635

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The c-Jun protein is a major component of the transcription factor AP-1, originally shown to mediate phorbol ester tumor promoter (TPA)-induced expression of responsive genes through the TPA-response element (TRE). The Jun proteins form homo- and heterodimers which bind the TRE, while Fos proteins are active only as heterodimers with any of the Jun proteins. Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. A distant member of the MAP kinase family, designated c-Jun NH2-terminal kinase (JNK1) functions to regulate c-Jun by phosphorylation at the amino terminal serine regulatory sites, Ser 63 and Ser 73). RPL10 has been described as a transcription factor that can function to bind DNA directly or alternatively can interact with c-Jun to inhibit transactivation of AP-1 promoter driven reporter vectors by Jun-Jun homodimers. RPL10 is highly conserved throughout eukaryotic evolution and is apparently a member of a multi-gene family.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: RPL10 (F34) polyclonal antibody detects endogenous levels of RPL10 protein.

Extra Notes: Western blot (WB) analysis of RPL10 (F34) pAb at 1:500 dilution
Lane1:C6 whole cell lysate(40ug)
Lane2:BV2 whole cell lysate(40ug)
Lane3:HEK293T whole cell lysate(40ug)
Lane4:A549 whole cell lysate(40ug)
Lane5:PC3 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 25 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: 60S ribosomal protein L10; Laminin receptor homolog; Protein QM; Tumor suppressor QM; DXS648E; QM

Immunogen: Synthetic peptide, corresponding to the N-terminal of Human RPL10.

Conjugate: Unconjugated

Modification: Unmodified