Thrombin R (G17) Polyclonal Antibody

Catalogue Numbers: BS1856-50, BS1856-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P25116

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Thrombin receptor (also designated protease-activated receptor-1 or PAR-1), PAR-2 and PAR-3 compose a distinct class of G protein-coupled receptors activated by proteolysis. Cleavage of these receptors by proteases occurs within the amino-terminal extracellular domain. Thrombin, a serine protease involved in platelet aggregation and blood coagulation, activates the thrombin receptor, resulting in elevated intracellular calcium levels in platelets. Thrombin also cleaves PAR-3 in vitro, suggesting that PAR-3 may be involved in thrombosis or mitogenesis. Thrombin receptor and PAR-4 appear to account for most thrombin signaling in platelets. Activation of PAR-2 in vitro is induced by trypsin, suggesting that PAR-2 is not an alternative thrombin receptor. Cytokines including TNF-α and IL-1β increase PAR-2 expression, indicating PAR-2 involvement in the acute inflammatory response.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Thrombin R (G17) polyclonal antibody detects endogenous levels of Thrombin R protein

Extra Notes: Western blot (WB) analysis of Thrombin R (G17) pAb at 1:500 dilution
Lane1:PC3 whole cell lysate(40ug)
Lane2:U-87MG whole cell lysate(40ug)
Lane3:The Brian tissue lysate of Rat(10ug)
Lane4:The Brain tissue lysate of Mouse(20ug)

Bioworld Molecular Weight: ~ 47 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Proteinase-activated receptor 1; PAR-1; Coagulation factor II receptor; Thrombin receptor; F2R; CF2R; PAR1; TR

Immunogen: Synthetic peptide, corresponding to the N-terminal of Human Thrombin R.

Conjugate: Unconjugated

Modification: Unmodified