NUR77 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90978-50, BS90978-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P22736(Human) P12813(Mouse) P22829(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: Nurr1 (Nur-related factor 1) and Nur77 (also designated NGFI-B) encode orphan nuclear receptors which may comprise an additional subfamily within the nuclear receptor superfamily. The rat and human homologs of mouse Nurr1 are designated RNR1 and NOT, respectively. Both Nurr1 and Nur77 are growth factor inducible immediate early response genes. Induction of both Nurr1 and Nur77 is seen after membrane depolarization while only Nur77 induction is seen with NGF stimulation. JunD acts as a mediator for Nur77. An increase in Nur77 expression is seen in activated T cells during G0 to G1 transition and throughout the G1 phase. In addition to its function as an immediate early gene, Nur77 may play a role in TCR-mediated apoptosis. Cyclosporin A, a potent immunosuppressant, has been shown to inhibit the ability of Nur77 to bind DNA. A dominant negative form of Nur77 can protect T cell hybridomas from activation-induced apoptosis. However, the absolute requirement of Nur77 for TCR-mediated apoptosis is still under debate.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: NUR77 polyclonal antibody detects endogenous levels of NUR77 protein.

Bioworld Molecular Weight: 64 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of NUR77 on rat brain cells lysates using anti-NUR77 antibody at 1/500 dilution., ICC staining NUR77 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Nur77, Early response protein NAK1, GFRP 1, GFRP, GFRP1, Growth factor inducible nuclear protein N10, Growth Factor Inducible Nuclear Protein NP10, Growth Factor Response Protein 1, Hbr1, HMR, Hormone Receptor, MGC9485, N10, N10 nuclear protein, NAK 1, NAK1, Nerve growth factor IB nuclear receptor variant 1, NGFIB, NP 10, NP10, NR4A1, NR4A1, Nuclear hormone receptor NUR/77, Nuclear Hormone Receptor TR3, Nuclear receptor subfamily 4 group A member 1, NUR77, NUR77, mouse, homolog of, Orphan nuclear receptor HMR, Orphan nuclear receptor NR4A1, Orphan nuclear receptor TR3, Orphan receptor tr3, Receptor NGFIB, ST 59, ST-59, ST59, Steroid receptor TR3, Testicular receptor 3, TR 3, TR3, TR3 orphan receptor,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified