VAMP2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91421-50, BS91421-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P63027(Human) P63044(Mouse) P63045(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IP, ICC/IF FC

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
FC:1:50-1:100

Background: Syntaxins were originally thought to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimide-sensitive factor), SNAP 25 (synaptosomal-associated protein of 25 kDa), SNAPs (soluble NSF attachment proteins) and synaptotagmin. VAMPs, also designated synaptobrevins, including VAMP-1 and VAMP-2, and synaptotagmin, a protein that may function as an inhibitor of exocytosis, are vesicular proteins. SNAPs, including a- and γ-SNAP, are cytoplasmic proteins that bind to a membrane receptor complex composed of VAMP, SNAP 25 and syntaxin. SNAPs mediate the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exo-cytosis by competing with SNAP 25 and syntaxins for VAMP binding.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: VAMP2 polyclonal antibody detects endogenous levels of VAMP2 protein.

Bioworld Molecular Weight: 15 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of VAMP2 on different cell lysates using anti-VAMP2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: SH-SY5Y
Lane 2: Jurkat, ICC staining VAMP2 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: VAMP-2, VAMP2, FLJ11460, RAt VAMPB, RAt VAMPIR, SYB, SYB2, Synaptobrevin 2, Synaptobrevin-2, VAMP 2, VAMP-2, Vamp2, VAMP2, Vesicle associated membrane protein 2, Vesicle-associated membrane protein 2 (synaptobrevin 2), Vesicle-associated membrane protein 2,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified