USP13 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91410-50, BS91410-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q92995(Human) Q5BKP2(Mouse)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC FC IHC

All Applications: WB:1:500-1:2,000
IHC/ICC:1:50-1:200
FC:1:50-1:100

Background: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP13 (ubiquitin specific peptidase 13), also known as ISOT3 (Isopeptidase T-3), is an 863 amino acid protein that belongs to the peptidase C19 family and contains one UBP-type zinc finger and two UBA domains. Highly expressed in testicular and ovarian tissue, USP13 functions to catalyze the water-dependent conversion of a ubiquitin C-terminal thioester to a thiol and a free ubiquitin.

Purification and Purity: Protein affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: USP13 polyclonal antibody detects endogenous levels of USP13 protein.

Bioworld Molecular Weight: 97 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of USP13 on different lysates using anti-USP13 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293T
Lane 2: Daudi
Lane 3: Mouse testis
Lane 4: Rat brain, ICC staining USP13 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: ISOT-3, USP13, ISOT3, Deubiquitinating enzyme 13, Isopeptidase T 3, Isopeptidase T-3, Isopeptidase T3, ISOT 3, ISOT-3, ISOT3, Ubiquitin carboxyl terminal hydrolase 13, Ubiquitin carboxyl-terminal hydrolase 13, Ubiquitin specific peptidase 13 (isopeptidase T3), Ubiquitin specific peptidase 13, Ubiquitin specific processing protease 13, Ubiquitin specific protease 13, Ubiquitin thioesterase 13, Ubiquitin thiolesterase 13, Ubiquitin-specific-processing protease 13, UBP13, UBP13, USP 13, USP13,

Immunogen: Recombinant protein within human USP13 aa 550-800.

Conjugate: Unconjugated

Modification: Unmodified