UBA1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91397-50, BS91397-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P22314(Human) Q02053(Mouse) Q5U300(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:100-1:500
FC:1:50-1:100

Background: The ubiquitin activating enzyme E1 (UBE1) catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. Specifically, UBE1 functions to adenylate the C-terminal glycine residue of ubiquitin, a reaction that is ATP-dependent and is proceeded by the formation of a thiolester bond with a cysteine residue of UBE1. The UBE1-activated ubiquitin is then transferred to a ubiquitin conjugated enzyme, which donates the ubiquitin residue to target substrates. The UBE1 gene is an example of an X-Y homologous gene, which is X-linked with a distinct Y-linked gene in many mammals. However, no UBE1 homolog is detectable on the human Y chromosome. UBE1 is thought to escape X inactivation in humans.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: UBA1 polyclonal antibody detects endogenous levels of UBA1 protein.

Bioworld Molecular Weight: 118 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of UBA1 on mouse liver lysates using anti-UBA1 antibody at 1/5,000 dilution., ICC staining UBA1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: UBA1, A1S9, A1S9 protein, A1S9T and BN75 temperature sensitivity complementing, A1S9T, A1ST, AMCX1, CFAP124, CTD-2522E6.1, GXP 1, GXP1, MGC4781, POC20, POC20 centriolar protein homolog, Protein A1S9, SMAX2, Uba1, UBA1, ubiquitin-activating enzyme E1 homolog A, UBA1, UBA1A, UBE 1, UBE 1X, UBE1, UBE1X, Ubiquitin activating enzyme E1, Ubiquitin-activating enzyme E1, Ubiquitin-like modifier-activating enzyme 1,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified